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Fig. 7. Effect of mutant GPI-anchored CEACAM1 protein on biochemical myogenic differentiation. Left column: phase contrast light photomicrographs; right column: corresponding anti-myosin antibody plus FITC-conjugated, anti-mouse IgG-stained fluorescent photomicrographs of cultures of various transfectant cells incubated for 4 days in differentiation medium. Cytoplasmic staining of multinucleated myotubes can be seen for L6(Neo) and L6(CC1-4L), indicating myosin synthesis and myogenic differentiation. Magnification, 600x.