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Fig. 4. Deletion analysis reveals multiple contributions to the membrane binding of ßH segment 33. Sagittal confocal sections of salivary glands expressing ßH33 and its derivatives (Fig. 1B) under the control of the 185Y-Gal4 driver. All images show costaining for the ßH derivative using the mAb 9E10 (left panel, red in merged image) and {alpha}-spectrin (right panel, green in merged image). Embryos are at stage 14-16. (A-C) Costaining for ßH33. (D-F) Costaining for ßH33{Delta}PH. (G-I) Costaining for ßHPH+3. (J-L) Costaining for ßHPH+5-3. Bars, 10 µm.