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Fig. 5. Immunohistochemical localization of protease inhibitor {alpha}2-MG in the seminiferous epithelium of rat testes at the BTB site during CdCl2-induced BTB damage. (A-D) Immunoreactive {alpha}2-MG in the seminiferous epithelium after treatment of rats with CdCl2 (3 mg/kg b.w., i.p.). Shown are different magnifications of cross sections of testes at time 0 hours, 20 hours, 24 hours and 96 hours. Immunoreactive {alpha}2-MG appears as reddish-brown precipitates. (E) Control in which normal rabbit serum was substituted for the primary antibody, illustrating that the staining for {alpha}2-MG shown in A-D is specific. The smaller images in A, B and E are higher magnifications of the representative micrographs shown on the left panel that illustrate the cellular localization of {alpha}2-MG in the seminiferous epithelium. Bars in left panel of A and E, 200 µm; bars in C, in middle panel of A and right panel of E, 80 µm; bar in right panel of A, 20 µm. Bars shown in A are applicable to B, and bar in C is applicable to D.