Fig. 6. Cytoskeletal requirements of dextran endocytosis and intracellular trafficking in osteoclasts. Osteoclasts settled overnight on dentine disks were incubated with vehicle (A), 10 µM cytochalasin D (B), 10 µM nocodazole (C) or 10 µM paclitaxel (D) for 30 minutes before the addition of TRITC-labelled dextran-3000 in the presence of the inhibitors. Representative lateral images of osteoclasts after 20 minutes of labelling are shown after immunodecoration with antibodies directed against the _vß₃ integrin (A-D, red indicates TRITC-labelled dextran and green indicates _vß₃ integrin); arrows indicate the position of the bone surface relative to the label. Scale bar, 10 µm. The number of osteoclasts with dextran endocytosis was counted and expressed as a percentage. Columns show mean percentage of osteoclasts with dextran endocytosis and s.e.m.