Fig. 3. mRNA expression of Ltbp-2, -3 and -4 in AhR–/– MEF. RNA expression for additional members of the LTBP family was performed by RT-PCR using total RNA and the gene-specific primers listed in Table 1. No significant differences were found in the expression of Ltbp-2 (A), Ltbp-3 (B) or Ltbp-4 (C) between AhR+/+ and AhR–/–. The expression of ß-actin was used to check for RNA quantitation and integrity. The experiment was performed by duplicate in at least two MEF preparations.