Fig. 7. Antibodies against LTBP-1 and TGFß decreased the inhibitory properties of conditioned medium from AhR–/– MEF. AhR–/– MEF were grown for 48 hours in the presence of 1 µg/ml anti-LTBP-1 (hinge) or 1 µg/ml anti-TGFß (1D-11) antibodies and conditioned medium prepared as indicated in Materials and Methods. (A) Conditioned media from cultures left untreated (No Ab), treated with anti-LTBP-1 (anti-LTBP) or anti-TGFß (anti-TGFß) were added to Mv1Lu cells and their inhibitory potential measured as indicated in Materials and Methods. (B) Using the same conditioned media; MMP-2 activity was determined by using a gelatinolytic assay. The experiments were performed in triplicate (A) or duplicate (B) in two MEF cultures.