Fig. 4. Comparison of the extent of neurofilament proteolysis between control and NFHLacZ transgenic samples. Neurofilaments purified from brain were digested at 30°C during increasing times (0-60 minutes) with 4 ng trypsin (A) or -chymotrypsin (B), and the intact subunits were revealed by western blot. Their amount was a proportion of the initial time (t=0). Means and standard deviations of a typical triplicate experiment are shown. Results for control mice are represented by black lanes and those for transgenic NFHLacZ mice by white lanes.