Fig. 2. Co-immunoprecipitation of MIP(AQP0) with embryonic lens fiber connexins. [³⁵S]-Methionine-labeled gap-junction-rich membranes isolated from embryonic-day-9 lenses were immunoprecipitated with affinity-purified anti-Cx45.6 (lanes 1 and 2) and anti-Cx56 (lanes 3 and 4) antibodies in the absence (lanes 2 and 4) or presence (lanes 1 and 3) of 0.6% SDS. Lens lysate was immunoblotted by anti-MIP(AQP0) antiserum (lane 5).