Fig. 4. Expression and colocalization of MIP(AQP0) and Cx45.6 detected by dual-immunostaining of coronal sections of embryonic-day-10 and -20 lens. (A) Coronal sections from bow (A-C) and core (D-F) regions of embryonic-day-10 chick lens were prepared and co-immunostained with antibodies specific for MIP(AQP0) and Cx45.6, and subsequently stained with fluorescein-conjugated goat anti-mouse IgG for MIP(AQP0) (A,D) and followed by rhodamine-conjugated goat anti-rabbit IgG for Cx45.6 (B,E). The corresponding images from the same regions were merged together to demonstrate the overlapping patterns between these two proteins (C,F). (B) Coronal sections from embryonic-day-20 chick lens were similarly prepared and co-immunostained with anti-MIP(AQP0) (A,D) and Cx45.6 (B,E) antibodies. The merged images were shown in (C,F). Scale bar, 20 µm.