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Fig. 6. C-terminus of MIP(AQP0) pulled down lens-fiber connexins from chick embryonic-day-10 lens lysates. Ni-NTA beads conjugated with six-histidine-tagged C-terminus of MIP(AQP0) were used to pull down proteins from lens lysates. Total lens lysates (lane 1) and retained fractions of lysate by Ni-NTA beads in the absence (lane 2) or presence (lane 3) of fusion protein were visualized by Coomassie Blue staining. The retained fraction of lysate by fusion-protein-conjugated-beads (lanes 4 and 7), the flow-through factions after extraction by fusion-protein/Ni-NTA beads (lanes 5 and 8) and total lens lysate samples (lanes 6 and 9) were immunoblotted with affinity-purified anti-Cx45.6 antibody (lanes 4-6) and anti-Cx56 antibody (lanes 7-9).