Fig. 3. Cin and Cin + pcnx1⁺ cells display temperature-sensitive growth. Cells were cultured to saturation and then diluted into 10 ml of fresh MM+Ade+Ura medium to OD₅₉₅ of 0.02. (A) Growth curves of SP556 (genomic cnx1⁺), SP3220: cnx1 + pcnx1⁺, SP7188: Cin and SP7202: Cin + pcnx1⁺ cells at 30°C for 45 hours. (B) Growth curves of the same cells grown at 37°C for 72 hours. (C) Histograms of calculated growth rates for each strain, at 30°C and 37°C.