Fig. 6. Overexpression of Cdk5-p35 increases NF phospho-epitopes in perikarya. Panels present DRG neurons 24 hours after transfection with a construct expressing GFP-M in the absence (–) or presence (+) of constructs that express Cdk5 and p35, followed by immunofluoresce analyses with RT97 followed by Texas-Red-conjugated secondary antibody. Transfected cells were localized by GFP fluorescence (under fluorescein optics) and RT97 immunoreactivity was then imaged under rhodamine optics. The graph shows the mean percentage of RT97 immunoreactivity (± standard deviation, expressed in arbitrary densitometric units) that had translocated into axons. Notice that perikaryal RT97 immunoreactivity (the result of increased levels of phosphorylated NF-H) was significantly (P<0.05) increased in response to the overexpression of Cdk5-p35.