Fig. 2. Germination of spores disrupted for the ini1⁺ gene. (A) Phase contrast images of spores derived from an ini1⁺/ini1 tetrad upon germination in YE medium. A 2:2 segregation pattern is observed. (B) DNA content of germinating haploid spores derived from ini1⁺/ini1 diploids grown in either the presence of uracil, to allow all spores to germinate (left), or in the absence of uracil, to restrict germination to only those spores deleted for ini1⁺ (right). The positions of 1C and 2C DNA content are indicated. Cells were collected at 2-hour intervals for 12 hours and processed for flow cytometry (FACS) analysis as previously described (Sazer, 1990).