Fig. 3. Cells depleted of ini1+ arrest in the G2/M phase of the cell cycle. (A) DAPI-stained
ini1 cells containing the wild-type ini1+ gene under the control of the thiamine repressible promoter Rep81 (
ini1), grown in the absence (left panel) or presence (right panel) of thiamine. (B) Cell number increase for
ini1 in the presence or absence of thiamine. (C) DNA content of the cells described in A, 12 hours after addition of thiamine.