Fig. 3. Role of LAT C-terminal tail in TCR-induced recruitment of LAT to the immune synapse. APCs labelled with the CellTracker^TM Orange were loaded for 20 minutes with 5 µg/ml SEE, mixed with LATWT-, LAT32-104- and LATCt-GFP T cells and monitored by time-lapse confocal microscopy. Images were taken at 10-second intervals. Representative images taken from the digital movies at the times indicated (seconds) are shown. White and black arrowheads point respectively to the initial site of contact and to the intracellular GFP-labelled compartment. Bar, 5 µm.