Fig. 2. The nuclear localization of transgenes containing the suHw insulator in functional or non-functional arrangements. (A) Confocal image of imaginal disc nuclei from a single-suHw (SS) transgenic larva, hybridized with Cy3-labeled lacZ DNA probes (red) and an anti-lamin antibody (green) (SS-Cy3 group). (B) Confocal image of a transgenic line with paired-suHw (DS) (DS-Cy3 group). (C-D) Histogram of foci distribution along the radius of the SS-Cy3 (C) and the DS-Cy3 group (D) in independent transgene lines (colored lines). 3D foci histograms of pooled samples are represented by the black lines. (E) Summary of foci distribution in a quartile diagram. The distance between FISH foci and lamin staining as a fraction of the nuclear radius for each sample group (colored bars) is compared with a model of randomly distributed samples (gray bar). (F) Foci histograms along the nuclear radius of each sample groups (colored lines) and the probability density function along the nuclear radius for the random distribution model (gray dashed line). (G) Diagram of cytological locations of insertion sites in selected transgenes. Small vertical lines represent transgene insertions at various locations in the three major chromosomes (black, gypsy; blue, NS; red, SS; green, DS). Insertion sites for individual lines are listed below.