Fig. 1. Remodelling of the microtubule network by intracellular S. typhimurium. Cells were infected for 10 hours with S. typhimurium and fixed with cold methanol. Bacteria (WT, green in merged images) were detected with an anti-LPS antibody and cytoskeletal proteins (red in merged images) were detected using either an antibody against ß-tubulin, or TRITC-phalloidin to visualize F-actin. (A) Confocal x/y plane micrographs showing tubulin accumulation around intracellular bacteria in HeLa cells (upper panel), murine elicited peritoneal macrophages (middle panel) and INT407 cells (lower panel). (B) x/z section generated from a confocal z-stack of an infected HeLa cell. (C) Confocal micrographs of representative cells treated with cytochalasin D (cyto D) (lower panel) or nocodazole (noc) (upper panel) showing F-actin and tubulin (red in merged images) around S. typhimurium (green in merged images). Scale bars: 5 µm. Dotted lines indicate cell perimeters.