Fig. 2. Cell-cycle analysis using the LSC2. (A) Using LSC2, cells can be sorted into cell-cycle stages (Kawasaki et al., 1997). Cells in red boxes are at prophase and metaphase, and those in blue boxes are at anaphase and telophase. (B) Topo II-knockdown, topo IIß-knockdown and mock-transfected cells were sorted using the LSC2 3 days after transfection (plots). The tables show the number of cells in each category; red boxes correspond to prophase/metaphase cells, blue boxes to anaphase/telophase cells, 2C to G2 phase cells and `total mitotic' is the sum of prophase/metaphase and anaphase/telophase cells. Each transfection was repeated independently at least three times and samples were prepared in four separate Akura films for every experiment.