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Fig. 3. Chromosome segregation occurs in topo II{alpha}-knockdown cells. (A,a-c) Three representatives in mitotic stages were depicted from mock-transfected cells. (B,a-f) Three days after transfection, topo II{alpha}-knockdown cells were stained with DAPI, anti-topo II{alpha} antibody (8D2) and anti-tubulin antibody (YL1/2). (C,a,b) Threads of DNA are indicated by arrowheads. (D) Topo II{alpha}-knockdown cells were also stained with anti-NPC (nuclear pore complex protein) antibody. (E) The number of nuclei connected by a thread of DNA was counted. Bar, 10 µm.