Fig. 2. Distribution of Sam68 in GFP-Sam68-transfected hippocampal neurons. (A) Quantification and representative images of the subcellular localization of GFP-Sam68 in nuclei (N), nuclei + soma (N+S) and in nuclei + soma + dendrites (N+S+D). Error bars represent s.d. (n=3, where 200-300 transfected cells were counted). (B) Nucleo-somatodendritic expression of the N-terminal deleted GFP-Sam68 mutant transfected neuron. (C) GFP-Sam68-transfected neuron where individual granules moving into dendrites of living hippocampal neurons could be visualized. Transfected neurons were incubated in control medium as described in Materials and Methods and individual motilities of green fluorescent granules were observed by time-lapse video microscopy for 8 hours each 20 minutes. Images taken at t=0 and t=480 minutes are shown. Arrows label moving granules, whereas the arrowhead labels a stationary granule.