Fig. 4. Translocation of GFP-Sam68 from the nucleus to the somato-dendritic compartment in depolarized hippocampal neurons. (A) GFP-Sam68CREs-transfected (left panel) or GFP-SC35CREs-transfected (right panel) neurons were incubated with 5 mM KCl (white) or with 25 mM KCl (hatched or stippled) for 5 hours. Quantification of the subcellular localization of GFP-Sam68 was then reported as a percentage of transfected neurons showing nuclei (N), nuclei + soma (N+S) and nuclei + soma + dendrites (N+S+D) localization (n=3, where 200-300 transfected cells were counted). (B) Patterns of expression of Sam68 in GFP-Sam68CREs- or GFP-SC35CREs-transfected neurons depolarized (25 mM KCl) or not (5 mM KCl) for 5 hours. (C) Tyrosine-phosphorylation pattern of neurons depolarized (25 mM KCl) or not (5 mM KCl) for 5 hours.