Fig. 6. Inhibition of Sam68 translocation during depolarization. (A) Neurons were depolarized with 25 mM KCl for 5 hours in the absence (–) or presence of nimodipine (5 µM) or leptomycin B (LMB, 60 ng/ml) to block calcium entry through voltage-sensitive calcium channels or CRM-1 nuclear export, respectively. s.d. with n=3, where 200-300 transfected cells were counted (percentages of neurons with nuclear and somatic Sam68 expression are not shown). (B) Images of neurons transfected with GFP-Rev-NES or GFP-Sam68CREs treated or not with LMB (60 ng/ml) for 5 hours.