Fig. 5. Effects of wortmannin on drug-induced disappearance of CEACAM1-L. Confluent, polarized MDCK cells expressing rat CEACAM1-L were incubated with (right column) or without (left column) 50 nM wortmannin for 30 minutes at 37°C. Cells were then not treated (A) or treated with 100 µM pervanadate for 5 minutes (B) or with 20 nM staurosporine for 2 hours (C). Cells were then analyzed for CEACAM1-L expression by confocal microscopy using antibody against rat CEACAM1. Wortmannin inhibited the CEACAM1-L lateral disappearance induced by pervanadate but not that induced by staurosporine. Prominent intracellular vesicular staining for CEACAM1-L is seen in the staurosporine-treated cells. A-C each show a z-section, and x,y-sections between the apical and basal surfaces of permeabilized cells.