Fig. 3. EB1 and its C-terminal binding partner APC co-purify with centrosomes. Centrosomes were purified from nocodazole-treated MDCK cells (a-o) or U-2 OS cells (a'-o'), stained for the centrosome markers -tubulin (green in a-c,a'-c'), centrin (red in d-f,d'-f') or pericentrin (red in g-n,g'-n') and co-stained for APC (red in a-c,a'-c' and green in d-f,d'-f'), EB1 (green in g-j,g'-j') or p150^Glued/dynactin (green in k-n,k'-n'). Most of the purified centrosomes contain APC and EB1 (white arrowheads in a-j,a'-j'). An example of a centrosome without APC is shown (black arrowhead in d-f). By contrast, most of the centrosomes have little p150^Glued/dynactin (arrows in k-n,k'-n') or no p150^Glued/dynactin (black arrowheads in k-n). Examples of centrosomes that contain p150^Glued/dynactin are shown (white arrowheads in k'-n'). Purified MDCK (o) or U-2 OS (o') centrosomes are functional as measured by induction of MT aster formation in vitro (o,o'). Bar, 10 µm.