Fig. 1. PDGFR-ß expression in the male urogenital ridge between 11.5 and 13.5 dcp. (A) Immunohistochemical detection of PDGFR-ß localization in cryostat sections of 11.5 and 13.5 dpc male urogenital ridges. At 11.5 dpc no immunoreactivity can be detected in the gonad (a), while at 13.5 dpc cells with a strong immunoreactivity fill the interstitial compartment of the gonad (c). (b,d) control sections. m, mesonephros; t, testis. Bar, 100 µm. (B) Immunoblot analysis of PDGFR-ß expression, showing the presence of a 190-kDa immunoreactive band that reacts to the same antibody as used for immunolocalization. The presence of equal amounts of proteins in each lane is confirmed by quantitatively similar ß-actin immunoreactive bands.