Fig. 6. Effect of PDGF-BB on mesenchymal mesonephric cell migration. Assays were performed by incubating cells for 16-18 hours in cell culture inserts with 8 µm porosity membrane. Cells that had migrated to the lower surface of the filters were counted. (A) Dose-response curve. Various concentrations of PDGF-BB were added to the lower wells only. (B) Chemotaxis vs chemokinesis analysis. PDGF-BB (10 ng/ml) was added to the upper well, to the lower well, or to both set of wells. Results are mean±s.d. of three independent experiments, each performed in duplicate. ^*P<0.01 vs untreated control.