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Fig. 6. Effects of TAL-1 knockdown on in vitro angiogenesis. HUVECs were transfected with tal-1 or control siRNA and analyzed 24 hours after the second transfection for TAL-1 protein content or in vitro angiogenesis. tal-1 silencing in endothelial cells by RNA interference. TAL-1 expression was assessed in whole cell lysates (30 µg) by immunoblotting with the anti-TAL-1 mAb 3BTL73. The blot was reprobed with an anti-GAPDH rabbit antiserum to control protein loading. Effect of TAL-1 knockdown on in vitro angiogenesis. Representative photographs of transfected HUVECs after 4 hours of plating on Matrigel.