Fig. 3. Frequencies of meiotic stages in wild-type, heterozygous and Lmna^–/– testes suspensions. Mid-preleptotene and bouquet frequencies are based on Maj.Sat/T₂AG₃ FISH (Scherthan et al., 1996) analysis of >1950 meiocytes/genotype. H1t⁺, histone H1t-expressing spermatocytes as identified by anti-H1t immunofluorescence (Scherthan et al., 2000a). All other stages were determined by SCP3 immunofluorescence analysis of at least 200 spermatocytes of each genotype. The absence of A-type lamins induces a highly significant increase in early prophase stages, while later stages (H1t⁺ late pachytene and diplotene) are significantly reduced, even in the heterozygote. ^*Highly significantly different (², P<0.001) and ^#significantly different (², P<0.01) compared with WT. ^aMid-preleptotene and bouquet values determined by Cen/Tel FISH (Scherthan et al. 2000a).