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Fig. 1. RNAi depletion of CP190 or CP60 does not lead to growth or mitotic defects in tissue culture cells. (A) A Western blot showing the depletion of CP190 and CP60 in mock (lane 1), CP60 (lane 2) or CP190 (lane 3) RNAi-treated cells 96 hours after treatment. (B) Graph showing the total number of cells per ml during the 5-day time course of a typical RNAi-depletion experiment. (C) Graph showing the mitotic index (as judged by number of phospho-histone H3-positive cells) at the 96-hour time point of a typical RNAi-depletion experiment. The total numbers of cells counted were 811, 969, and 1052 for the control, CP60, and CP190 RNAi experiments, respectively. Similar results were obtained in two separate RNAi experiments (data not shown).