Fig. 8. The overexpression of CP190 or CP190M is lethal, but this lethality can be rescued by the co-overexpression of CP60. (A) A western blot showing the levels of CP190 or CP190M in wild-type adults (lane 1), in three independent homozygous transgenic Pubq-CP190 lines (lanes 2-4), and in three independent heterozygous Pubq-CP190M transgenic lines. All three Pubq-CP190 transgenic lines are homozygous viable, but exhibit high levels of pupal mortality, whereas all three Pubq-CP190M lines are homozygous lethal. (B) A western blot showing the levels of CP190, CP190M or CP60 in wild-type adults (lane 1), or in adults that are homozygous for independent Pubq-CP190 insertions and a Pubq-CP60 insertion (lanes 2,3), or in adults homozygous for independent Pubq-CP190M insertions and a Pubq CP60 insertion (lanes 4,5). Note that both the Pubq-CP190 insertions and both the Pubq-CP190M insertions used in this experiment are normally homozygous lethal. The homozygous adults appear to be viable because they also overexpress CP60. The asterisk highlights a background band that is recognised by the anti-CP60 antibodies and is shown here as a loading control.