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Fig. 4. hFis1 is targeted to the mitochondria via basic residues within its C-segment and its transmembrane domain. (A) Schematic representation of GFP-hFis1 fusion proteins and their subcellular location. (B-F) COS-7 cells expressing the GFP-hFis1 fusion proteins depicted in (A) were incubated with MitoTracker Red and the nuclear stain Hoechst 33258 before analysis by fluorescence microscopy.