Fig. 6. Complementation studies in S. cerevisiae. (A) Yeast fis1 cells expressing a mitochondrial targeted from of GFP as well as either vector alone (p-empty), vector expressing yeast Fis1p or hFis1 were subjected to DIC and fluorescence microscopy. (B) GFP-hFis1 is mis-targeted to the ER in yeast cells. By contrast, GFP-hFis1(N)-yFis1p(TM), a chimeric fusion protein containing the N-terminal cytosolic domain of hFis1 and the transmembrane domain and C-segment of yeast Fis1p, is targeted to the mitochondria but fails to complement in fis1 cells.