Fig. 2. Effect of EGF on Cx43 localization in IAR20 cells. IAR20 cells were either left untreated (A) or treated with 50 ng ml^–1 EGF for 30 minutes (B) or 60 minutes (C). (D) IAR20 cells were preincubated with 50 µM PD98059 for 30 minutes, then incubated with EGF at a final concentration of 50 ng ml^–1 and incubated for 30 minutes in the sustained presence of PD98059. The cells were fixed, immunostained with anti-Cx43 antibodies and visualized using fluorescence microscopy.