Fig. 3. Effects of cycloheximide and MG132 on EGF-induced relocalization of Cx43. IAR20 cells were either left untreated (A) or treated with 50 ng ml^–1 EGF for 30 minutes (B). (C) Cells were preincubated with 10 µg ml^–1 cycloheximide for 2 hours, then exposed to EGF at a final concentration of 50 ng ml^–1 and incubated for 30 minutes in the sustained presence of cycloheximide. (D) Cells were preincubated with 10 µM MG132 for 30 minutes, then exposed to EGF at a final concentration of 50 ng ml^–1 and incubated for 30 minutes in the sustained presence of MG132. Cells were fixed, immunostained with anti-Cx43 antibodies and visualized using fluorescence microscopy.