Fig. 7. Effect of hypertonic sucrose medium on EGF-induced internalization of Cx43. IAR20 cells were incubated with 50 ng ml^–1 EGF for 45 minutes at 37°C in Hepes-buffered DMEM without (A) or with (B) 0.45 M sucrose. The cells were fixed, immunostained with anti-Cx43 antibodies and visualized using fluorescence microscopy. Cells were incubated with 50 µg ml^–1 Alexa-488-transferrin in the absence (C) or presence (D) of 0.45 M sucrose for 45 minutes at 37°C. The cells were fixed and transferrin was visualized using fluorescence microscopy.