Fig. 12. Intercellular motility and endocytosis of TJs. (A) Confluent cultures of Eph4:NGFP-Cld3 cells on coverslips were wounded by manually scratching with a needle. After 2 hours culture, the dynamic behavior of GFP-positive TJs in the third row of cells from the front of the wound (lower panel) was compared with that of cells in the nonwounded sheets (upper panel). In the wounded sheet, cells moved upwards (arrow). Elapsed time is indicated at the bottom (in minutes:seconds). (B,C) The number of GFP-positive granules per cell was counted in the nonwounded sheets and in cells in the third row (asterisks in B; phase-contrast image) of the wounded sheet. Bars, 20 µm.