Fig. 3. Time-lapse observation of the remodeling of GFP-positive TJs in Eph4:NGFP-Cld3 cells under confluent conditions. Elapsed time is indicated at the bottom (in minutes:seconds). (A) Shortening of TJs between adjacent cells. Owing to intercellular motility, TJs (between arrows) decreased in length during a 120 minute period. (B) Close time-lapse observation of shortening TJs. GFP-positive granules budded from the TJ and moved into the cytoplasm (arrowheads). See Movie 2 (http://jcs.biologists.org/supplemental/). Bars, 10 µm (A); 10 µm (B).