Fig. 6. Time-lapse observation of the process of cell detachment of adjoining CFP-claudin-1- and YFP-claudin-1-expressing MDCK cells. As these cells were mix-cultured at a low cell density, they were dissociated as a result of their motility. Before detachment, the TJ between two cells looked yellow, because both CFP- and YFP-claudin-1 contributed to this TJ. Even after detachment, many of the torn-off fragments of TJs on individual cells also looked yellow (arrows). Elapsed time is indicated at the bottom (in minutes:seconds). See Movie 4 (http://jcs.biologists.org/supplemental/). Bar, 10 µm.