Fig. 8. Internalized claudins and endosome markers. MDCK cells expressing GFP-claludin-4 were stained with anti-EEA1 (early endosome antigen 1) mAb (A) or antibodies specific for late endosome markers such as LBPA (lysobisphosphatidic acid) and Rab7 (B). GFP-positive claudin-4-containing vesicles did not carry EEA1, whereas some vesicles were positive for late endosome markers. The boxed areas in the left panels are enlarged in the right three panels. Bars, 10 µm.