Fig. 3. Rac1^–/– BMMs have altered morphology. (A) Growing BMMs derived from Wt and Rac1^–/– mice were stained for F-actin with TRITC-phalloidin. Scale bar: 10 µm. (B) Quantification of adhesive area. Growing Wt, WtCre and Rac1^–/– cells were stained for F-actin, and total area of adhesion was measured (see Materials and Methods). The results shown are mean ± s.e.m. of 100 cells from each population over three separate experiments. (C) Quantification of cell shape. Growing Wt, WtCre and Rac1^–/– cells were stained for F-actin, and elongation ratio (ratio of the longest to the shortest cell axes) was measured (see Materials and Methods). The results shown are mean ± s.e.m. of 100 cells from each population over three separate experiments. Statistical significance compared to Wt values was calculated using Student's t-test, ^***P<0.001.