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Fig. 3. (A) Subcellular distribution of 14-3-3{sigma}-YFP and 14-3-3{zeta}-YFP in the indicated mammalian cells. (B) HaCaT and Hela cells immunostained for endogenous 14-3-3{sigma} and 14-3-3{zeta}. (C) Quantitative analysis of the subcellular distribution of 14-3-3{sigma} (red) and 14-3-3{zeta} (blue). Solid bars represent the average nuclear/cytoplasmic (FN/FC) ratio obtained with YFP-labelled 14-3-3 isoforms. Hatched bars represent the quantitative analysis of immunolocalisations of endogenous 14-3-3{sigma} and {zeta}. Standard deviations are indicated (black lines).