Fig. 4. mßCD and genistein block CTX delivery to the Golgi but not to endosomes. HeLa cells were pretreated with either 5 mM mßCD for 30 minutes (A-D) or 100 µg/ml genistein for 30 minutes (E-H), then incubated with CTX-FITC (A,E) for 30 minutes at 37°C. The Golgi apparatus of a high-GM1-expressing HeLa cell is labeled with GM130 (B,F) and endosomes with TfR (C,G). Merged confocal images (D,H) present CTX-FITC in green, GM130 in red and TfR in blue with colocalization between CTX-FITC and GM130 in yellow and between CTX-FITC and TfR in cyan. Scale bar: 8 µm.