Fig. 1. Inhibition of adhesion by ß₃ cytoplasmic tail peptido-mimetics. K_Vß₃ WT or CSß₃ (A) and murine BMM or human MDM (B) cells were adhered to vitronectin in the presence (PMA) or absence (control) of PMA (10 ng/ml), and either anti-ß₃ antibody (5 µg/mL), TAT-Yp (10 mM) or TAT-Y/F (10 mM) fusion peptides for 1 hour at 37°C as described in Materials and Methods. Data represent the mean±s.d. from triplicate wells from three separate experiments, presented as percent adhesion. (C) K562 cells expressing wild-type, Y747F, Y759F or Y747,759F mutant _Vß₃ were incubated without (untreated) or with MnCl (2 mM) and sodium pervanadate (100 µM) for 10 minutes at 37°C. Whole cell lysates and ß₃ immunoprecipitates were separated by SDS-PAGE and western blotted with anti-ß₃ Y747 PSSA and HRP-conjugated secondary antibody or secondary alone (2° alone).