JCS -- Chandhoke et al. 117 (8): 1431 Figure IG3

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Fig. 3. Sustained ß₃ Y747 phosphorylation during ROCK inhibition. K ${alpha}$ _Vß₃ WT cells were adhered to vitronectin-coated coverslips in the presence of Rho kinase (ROCK) inhibitor, Y27632 (10 µM) (A-C) or Y27632 and PMA (10 ng/ml) (D-F) for 10 (A,D), 30 (B,E) or 70 (C,F) minutes. Cells were stained for ß₃ Y747 PSSA and FITC-conjugated secondary antibody as described in Materials and Methods. Bar, 5 µm.