Fig. 1. Stage-specific incorporation of ¹⁴C-labeled oleic acid into TAG in P. falciparum-infected erythrocytes. Tightly synchronized cultures of Honduras-1 were labeled and total lipids associated with infected erythrocyte were analysed. (A) TLC of the extracted total lipid species for neutral (left) and polar (right) lipids. The positions corresponding to the authentic cold lipid species are indicated: CE, cholesteryl ester; DAG, diacylglycerol; FFA, free fatty acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; TAG, triacylglycerol. (B) Kinetics of the accumulation of various lipid species incorporated with radiolabeled oleic acid in P. falciparum-infected erythrocytes during intraerythrocytic development. At different time points, the distribution of lipid-associated radioactivity in TAG (filled circles), PC (open circles) and PE (open triangles) are shown. Values are the total radioactivity of each lipid from 5 ml of 3% hematocrit culture. At the top is shown the dominant parasite morphology at various sampling times. One of four independent experiments displaying similar profile is shown.