Fig. 6. Inhibition in the trafficking of Nile Red fluorescent lipid bodies with BFA. Tightly synchronized cultures of 3D7 were treated with 5 µg ml^-1 BFA or 0.1% ethanol (solvent control) for 0-26 hours (A) or 0-30 hours (B), or re-incubated for another 8 hours after BFA treatment (C). BFA inhibition of lipid body trafficking was visualized in live parasites (a) in comparison with the corresponding control culture (b). From left to right, panels represent bright-field images, DAPI signals (blue), Nile Red staining patterns (green) and merged images of DAPI and Nile Red. White areas denote regions of co-localization. Scale bar, 2 µm.