Fig. 8. DGAT activity in P. falciparum-infected erythrocyte ghosts and in isolated parasites. (A) Time dependence of TAG synthesis in P. falciparum-infected erythrocyte ghosts. TAG accumulation was monitored for 0-15 minutes from 50 µg infected erythrocyte ghost lysate at 20 mM Mg²⁺. (B) Dependence of DGAT activity on protein concentration. TAG synthesis rate using 0-50 µg infected erythrocyte ghost lysate at 20 mM Mg²⁺ was measured. (C) Mg²⁺ dependency of the DGAT activity associated with isolated parasite cells. DGAT activity at various concentration of Mg²⁺ (0-100 mM) using 6.0 µg lysate prepared from trophozoite/schizont-rich cultures by saponin treatment and N₂ cavitation was determined. (A-C) The reaction mixtures with and without 1,2-DAG are indicated by filled circles and filled triangles, respectively; values are averages of triplicates; and s.d. for each data is indicated by error bar.