Fig. 4. Expression of MLCK truncations increases the number of multinucleate cells. (A) HeLa cells were transiently transfected with GFP or with GFP fusions with the rabbit five DXRs, the HeLa IgG domain, the HeLa IgG domain plus five DXRs, MyBP-H or MyBP-C. Cells expressing GFP or GFP fusions were visualized by fluorescence. Values represent the mean and the standard error of the mean for 3000 cells per MLCK construct and 1000 cells per MyBP construct from three independent experiments. (B) Representative field of cells transfected with the IgG domain alone. Cells were fixed at 32 hours after transfection and stained with DAPI for DNA and anti-ß-tubulin antibodies. Cells expressing the IgG domain GFP fusion were visualized by green fluorescence. Arrows indicate cells with multiple nuclei. Scale bar, 10 µm.