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Fig. 7. Ct-CRM1 and Ct-Ran are associated with the BR mRNP particles during transport through the interchromatin. (A,B) BR mRNP particles (arrows) in the interchromatin labelled by Ct-CRM1-specific gold particles. (C,D) Ct-CRM1-labelled BR mRNP particles (arrows) docked at the NPCs. (E, F) BR mRNP particles (arrows) in the interchromatin, labelled by the anti-Ran antibody. (G,H) BR mRNP particles (arrows) docked at the NPCs, labelled by the anti-Ran antibody. To assist interpretation, schematic representations are shown below each electron micrograph. The labelled BR mRNP is shown in grey and the gold label in black. The nuclear membrane and the NPCs with their basket structure are outlined. Cyt, cytoplasm; Nu, nucleus. Bars, 100 nm.