JCS -- Lu et al. 117 (8): 1567 Figure IG1

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Fig. 1. (A) The effect of pertussis toxin on PAF-induced Ca²⁺ transients in two-cell embryos. Traces show the mean [Ca²⁺]_i from the number of embryos shown in brackets. The experiments were repeated three times. Embryos were pretreated with the concentration of pertussis indicated and then challenged with 37 nM PAF/ml. (B) The role of the PAF-receptor in PAF-induced calcium transients in two-cell embryos and expression of receptor in human embryos. Two-cell embryos collected from either Pafr+/+ or Pafr–/– parents were exposed to rPAF acetylhydrolase and then challenged with 20 ng PAF/ml. Traces are representative of over 30 embryos from three replicates.